Fred Chang, PhD, MD | Department of Cell & Tissue Biology

Professor

Cell and Tissue Biology

+1 415 476-8922

The Chang Lab studies cell morphogenesis and cell division. We are interested in fundamental questions concerning spatial organization within a single cell: How do cellular components organize to form cells of a specific size and shape? How might cells sense their own shape and size? Topics include: cytokinesis, placement of the cell division plane, cell polarity, dynamics of microtubules and actin, biomechanics of cell morphogenesis, and sensing of cell shape and size. Many of our studies are on the simple rod-shaped fission yeast Schizosaccharomyces pombe.

Publications

Intracellular diffusion in the cytoplasm increases with cell size in fission yeast.

Molecular biology of the cell

Tan C, Lanz MC, Swaffer M, Skotheim J, Chang F

Quantifying turgor pressure in budding and fission yeasts based upon osmotic properties.

Molecular biology of the cell

Lemière J, Chang F

Vast heterogeneity in cytoplasmic diffusion rates revealed by nanorheology and Doppelgänger simulations.

Biophysical Journal

Garner RM, Molines AT, Theriot JA, Chang F

Control of nuclear size by osmotic forces in Schizosaccharomyces pombe.

eLife

Lemière J, Real-Calderon P, Holt LJ, Fai TG, Chang F

Physical properties of the cytoplasm modulate the rates of microtubule polymerization and depolymerization.

Developmental cell

Molines AT, Lemière J, Gazzola M, Steinmark IE, Edrington CH, Hsu CT, Real-Calderon P, Suhling K, Goshima G, Holt LJ, Thery M, Brouhard GJ, Chang F

Starvation induces shrinkage of the bacterial cytoplasm.

Proceedings of the National Academy of Sciences of the United States of America

Shi H, Westfall CS, Kao J, Odermatt PD, Anderson SE, Cesar S, Sievert M, Moore J, Gonzalez CG, Zhang L, Elias JE, Chang F, Huang KC, Levin PA

Variations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast.

eLife

Odermatt PD, Miettinen TP, Lemiere J, Kang JH, Bostan E, Manalis SR, Huang KC, Chang F

Precise regulation of the relative rates of surface area and volume synthesis in bacterial cells growing in dynamic environments.

Nature communications

Shi H, Hu Y, Odermatt PD, Gonzalez CG, Zhang L, Elias JE, Chang F, Huang KC

Decoupling of Rates of Protein Synthesis from Cell Expansion Leads to Supergrowth.

Cell systems

Knapp BD, Odermatt P, Rojas ER, Cheng W, He X, Huang KC, Chang F

Reassessment of the Basis of Cell Size Control Based on Analysis of Cell-to-Cell Variability.

Biophysical Journal

Facchetti G, Knapp B, Chang F, Howard M

Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast.

Molecular biology of the cell

Zhurinsky J, Salas-Pino S, Iglesias-Romero AB, Torres-Mendez A, Knapp B, Flor-Parra I, Wang J, Bao K, Jia S, Chang F, Daga RR

Microtubule polymerase and processive plus-end tracking functions originate from distinct features within TOG domain arrays.

Molecular biology of the cell

Cook BD, Chang F, Flor-Parra I, Al-Bassam J

Reprogramming Cdr2-Dependent Geometry-Based Cell Size Control in Fission Yeast.

Current biology : CB

Facchetti G, Knapp B, Flor-Parra I, Chang F, Howard M

Structural basis of tubulin recruitment and assembly by microtubule polymerases with tumor overexpressed gene (TOG) domain arrays.

eLife

Nithianantham S, Cook BD, Beans M, Guo F, Chang F, Al-Bassam J

Who's Your DadA? d-Alanine Levels Regulate Bacterial Stiffness.

mBio

Odermatt PD, Arjes HA, Chang F, Huang KC

The outer membrane is an essential load-bearing element in Gram-negative bacteria.

Nature

Rojas ER, Billings G, Odermatt PD, Auer GK, Zhu L, Miguel A, Chang F, Weibel DB, Theriot JA, Huang KC

The XMAP215 Ortholog Alp14 Promotes Microtubule Nucleation in Fission Yeast.

Current biology : CB

Flor-Parra I, Iglesias-Romero AB, Chang F

Forces that shape fission yeast cells.

Molecular biology of the cell

Chang F

Morphogenesis of the Fission Yeast Cell through Cell Wall Expansion.

Current biology : CB

Atilgan E, Magidson V, Khodjakov A, Chang F

Electrochemical regulation of budding yeast polarity.

PLoS biology

Haupt A, Campetelli A, Bonazzi D, Piel M, Chang F, Minc N

The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis.

Molecular biology of the cell

Zhou Z, Munteanu EL, He J, Ursell T, Bathe M, Huang KC, Chang F

How and why cells grow as rods.

BMC biology

Chang F, Huang KC

Electrochemical control of cell and tissue polarity.

Annual review of cell and developmental biology

Chang F, Minc N

Mechanical Forces of Fission Yeast Growth.

Current biology : CB

Minc N, Boudaoud A, Chang F

Cortical regulation of cell size by a sizer cdr2p.

eLife

Pan KZ, Saunders TE, Flor-Parra I, Howard M, Chang F

Role of turgor pressure in endocytosis in fission yeast.

Molecular biology of the cell

Basu R, Munteanu EL, Chang F

Manipulating cell shape by placing cells into micro-fabricated chambers.

Methods in molecular biology (Clifton, N.J.)

Chang F, Atilgan E, Burgess D, Minc N

Csi1 links centromeres to the nuclear envelope for centromere clustering.

The Journal of cell biology

Hou H, Zhou Z, Wang Y, Wang J, Kallgren SP, Kurchuk T, Miller EA, Chang F, Jia S

Myosin Vs organize actin cables in fission yeast.

Molecular biology of the cell

Lo Presti L, Chang F, Martin SG

Localization of cytokinesis factors to the future cell division site by microtubule-dependent transport.

Cytoskeleton (Hoboken, N.J.)

Atilgan E, Burgess D, Chang F

Contributions of turgor pressure, the contractile ring, and septum assembly to forces in cytokinesis in fission yeast.

Current biology : CB

Proctor SA, Minc N, Boudaoud A, Chang F

Fission yeast Alp14 is a dose-dependent plus end-tracking microtubule polymerase.

Molecular biology of the cell

Al-Bassam J, Kim H, Flor-Parra I, Lal N, Velji H, Chang F

Noise reduction in the intracellular pom1p gradient by a dynamic clustering mechanism.

Developmental cell

Saunders TE, Pan KZ, Angel A, Guan Y, Shah JV, Howard M, Chang F

Characterization of dip1p reveals a switch in Arp2/3-dependent actin assembly for fission yeast endocytosis.

Current biology : CB

Basu R, Chang F

Influence of cell geometry on division-plane positioning.

Cell

Minc N, Burgess D, Chang F

CLASP promotes microtubule rescue by recruiting tubulin dimers to the microtubule.

Developmental cell

Al-Bassam J, Kim H, Brouhard G, van Oijen A, Harrison SC, Chang F

Electrical control of cell polarization in the fission yeast Schizosaccharomyces pombe.

Current biology : CB

Minc N, Chang F

Regulation of cytokinesis by the formin cdc12p.

Current biology : CB

Yonetani A, Chang F

Cytokinesis and the contractile ring in fission yeast: towards a systems-level understanding.

Trends in microbiology

Bathe M, Chang F

Cell-cycle control: don't supersize me.

Current biology : CB

Pan KZ, Chang F

Shaping fission yeast with microtubules.

Cold Spring Harbor perspectives in biology

Chang F, Martin SG

Mechanical forces of fission yeast growth.

Current biology : CB

Minc N, Boudaoud A, Chang F

Establishing new sites of polarization by microtubules.

Current biology : CB

Minc N, Bratman SV, Basu R, Chang F

Mechanisms for maintaining microtubule bundles.

Trends in cell biology

Bratman SV, Chang F

Regulation and targeting of the fission yeast formin cdc12p in cytokinesis.

Molecular biology of the cell

Yonetani A, Lustig RJ, Moseley JB, Takeda T, Goode BL, Chang F

Stabilization of overlapping microtubules by fission yeast CLASP.

Developmental cell

Bratman SV, Chang F

Regulation of the formin for3p by cdc42p and bud6p.

Molecular biology of the cell

Martin SG, Rincón SA, Basu R, Pérez P, Chang F

Shaping the actin cytoskeleton using microtubule tips.

Current opinion in cell biology

Basu R, Chang F

The cell-end factor pom1p inhibits mid1p in specification of the cell division plane in fission yeast.

Current biology : CB

Padte NN, Martin SG, Howard M, Chang F

Self-organization of microtubule bundles in anucleate fission yeast cells.

Nature cell biology

Daga RR, Lee KG, Bratman S, Salas-Pino S, Chang F

Asymmetric microtubule pushing forces in nuclear centering.

Current biology : CB

Daga RR, Yonetani A, Chang F

Regulation of cytokinesis by spindle-pole bodies.

Nature cell biology

Magidson V, Chang F, Khodjakov A

Dynamics of the formin for3p in actin cable assembly.

Current biology : CB

Martin SG, Chang F

New end take off: regulating cell polarity during the fission yeast cell cycle.

Cell cycle (Georgetown, Tex.)

Martin SG, Chang F

Role of fission yeast myosin I in organization of sterol-rich membrane domains.

Current biology : CB

Takeda T, Chang F

Dynamic positioning of the fission yeast cell division plane.

Proceedings of the National Academy of Sciences of the United States of America

Daga RR, Chang F

Tea4p links microtubule plus ends with the formin for3p in the establishment of cell polarity.

Developmental cell

Martin SG, McDonald WH, Yates JR, Chang F

Effects of {gamma}-tubulin complex proteins on microtubule nucleation and catastrophe in fission yeast.

Molecular biology of the cell

Zimmerman S, Chang F

Site selection for the cleavage furrow at cytokinesis.

Trends in cell biology

Burgess DR, Chang F

Regulation of actin assembly by microtubules in fission yeast cell polarity.

Novartis Foundation symposium

Chang F, Feierbach B, Martin S

Intra-nuclear microtubules and a mitotic spindle orientation checkpoint.

Nature cell biology

Zimmerman S, Daga RR, Chang F

Organization of a sterol-rich membrane domain by cdc15p during cytokinesis in fission yeast.

Nature cell biology

Takeda T, Kawate T, Chang F

Laser microsurgery in fission yeast; role of the mitotic spindle midzone in anaphase B.

Current biology : CB

Khodjakov A, La Terra S, Chang F

Imaging green fluorescent protein fusions in living fission yeast cells.

Methods (San Diego, Calif.)

Tran PT, Paoletti A, Chang F

Regulation of a formin complex by the microtubule plus end protein tea1p.

The Journal of cell biology

Feierbach B, Verde F, Chang F

Rsp1p, a J domain protein required for disassembly and assembly of microtubule organizing centers during the fission yeast cell cycle.

Developmental cell

Zimmerman S, Tran PT, Daga RR, Niwa O, Chang F

Analysis of mid1p, a protein required for placement of the cell division site, reveals a link between the nucleus and the cell surface in fission yeast.

Molecular biology of the cell

Paoletti A, Chang F

cdc12p, a protein required for cytokinesis in fission yeast, is a component of the cell division ring and interacts with profilin.

The Journal of cell biology

Chang F, Drubin D, Nurse P

How fission yeast fission in the middle.

Cell

Chang F, Nurse P

Isolation and characterization of fission yeast mutants defective in the assembly and placement of the contractile actin ring.

Journal of cell science

Chang F, Woollard A, Nurse P

Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2.

Cell

Chang F, Herskowitz I

CDC36 and CDC39 are negative elements in the signal transduction pathway of yeast.

Cell regulation

Neiman AM, Chang F, Komachi K, Herskowitz I